Top 12 Venture Capital Firms for B2B Startups To Knw

For founders preparing to raise capital, knowing which investors focus on B2B is a critical first step. The right partner understands the specific cycles of business-to-business sales and the nuances of enterprise technology or SaaS models.

To help you identify potential partners, our team at Rho curated this guide to some of the top venture capital firms investing in B2B. Use it to quickly get familiar with investors who could be a great fit for your company.

Just raised, or about to? Set up your financial stack with Rho in minutes. We provide business banking, corporate cards, and bill pay—built for startup teams moving fast.

Which VC Firms in B2B Are Right for Your Stage?

Whether your company is in its early stages or preparing for an exit, connecting with the right investors is key. This is a quick overview of B2B venture capital firms and the stages where they invest.

Pre-seed and Seed VC Firms in B2B

Pre-seed and seed funding is the earliest capital you'll raise, typically used to validate your idea and build an initial product. Some B2B software venture capital firms focusing on this stage include New York-based Work-Bench, 2048 Ventures, and Zane Venture Fund.

Early Stage VC Firms in B2B

Early-stage funding, which includes Series A and B rounds, is for companies with proven traction that need capital to scale their team and accelerate growth. Enterprise-focused venture capital firms like Storm Ventures, Mighty Capital, and Cervin Ventures often invest at this point.

Late Stage VC Firms in B2B

Late-stage capital supports mature companies as they expand into new markets, make acquisitions, or prepare for an exit like an IPO or M&A. While many firms invest across stages, investors such as MissionOG and Wildcat provide capital to B2B technology companies in their growth and later stages.

Keep in mind that some VC firms invest across all startup funding stages, too.

To help you find the right partner, we've gathered information on leading VC firms that invest in business-to-business companies. The list below covers their investment stages, geographic focus, and what makes each firm distinct for founders.

1. Work-Bench

Based in New York City, Work-Bench is a venture capital firm that invests in enterprise software companies, primarily at the seed stage. They focus on B2B startups tackling complex problems for large organizations.

The firm is known for backing future enterprise unicorns, with notable investments including digital identity company Socure and mental health platform Spring Health. Their portfolio shows a clear preference for companies building foundational B2B technology, from infrastructure like Cockroach Labs to vertical SaaS like VTS.

Work-Bench could be a great partner if you are an early-stage founder building for the enterprise market, particularly if you are based in New York. Their track record suggests a strong fit for founders with deep technical expertise and a clear vision for industry-wide impact.

• Investment stages: Seed and early-stage
• Industries of focus: Enterprise software, B2B, SaaS, financial services
• Geographical presence: New York City
• Founded: 2013
• Notable portfolio companies: Socure, Spring Health, Dialpad, Cockroach Labs
• Number of investments: 76

You can refer to their website here.

2. MissionOG

MissionOG is a Philadelphia-based venture capital firm that invests in B2B technology companies from early to growth stages. They provide capital to support businesses as they mature and expand their operations.

The firm shows a clear interest in financial services and B2B software, often leading or co-leading funding rounds. Notable investments include fundraising software provider iDonate and AI fraud-detection company Featurespace.

MissionOG is a suitable partner for founders of B2B technology companies that have already found product-market fit and are ready to scale. Their focus on financial services makes them a strong choice for fintech founders seeking growth capital.

• Investment stages: Early and late stage venture
• Industries of focus: B2B, financial services
• Geographical presence: Philadelphia, Pennsylvania
• Founded: 2012
• Notable portfolio companies: Venminder, Pay4Later (Deko), iDonate, Featurespace, Autobooks
• Number of investments: 68

You can refer to their website here.

3. Storm Ventures

Storm Ventures is a venture capital firm based in Menlo Park, California, that concentrates on early-stage enterprise software companies. They have a long history of backing B2B startups since their founding in 1997.

The firm has a track record of identifying future market leaders, with portfolio companies like marketing automation pioneer Marketo and cloud contact-center Talkdesk. Storm's early backing helped Talkdesk scale to a $10 billion valuation, demonstrating their expertise in scaling enterprise technology companies.

Storm Ventures could be an excellent partner if you are an early-stage founder building an enterprise software solution. Their deep focus and history of guiding companies to significant exits make them a strong choice for teams with large-scale ambitions.

• Investment stages: Seed, early, and late stage
• Industries of focus: B2B, enterprise software, financial services, fintech
• Geographical presence: Menlo Park, California
• Founded: 1997
• Notable portfolio companies: Talkdesk, Pipedrive, Marketo, MobileIron
• Number of investments: 278

You can refer to their website here.

4. Mighty Capital

Mighty Capital is an early-growth venture capital firm in San Francisco that centers its investment thesis on product management. They back companies where strong product leadership is a key driver of business success.

The firm’s product-centric approach is evident in its portfolio, which includes high-profile exits like the home-sharing marketplace Airbnb and product-analytics platform Amplitude. Their focus on product-led companies signals an interest in businesses that win through superior user experience and innovation.

Mighty Capital is a strong match for founders with a product-led growth model who are entering their early growth phase. If you prioritize product as your core differentiator, this firm could be the right partner to help you scale.

• Investment stages: Early Stage Venture, Seed, Venture
• Industries of focus: B2B, Enterprise Software, Product Management
• Geographical presence: San Francisco, California
• Founded: 2017
• Notable portfolio companies: Airbnb, Amplitude, DigitalOcean, Cameo, Netskope
• Number of investments: 33

You can refer to their website here.

5. Zane Venture Fund

Zane Venture Fund is an Atlanta-based firm that invests in high-growth startups led by diverse and overlooked founders. Their mission is to provide capital to entrepreneurs building tech-enabled solutions.

The firm's focus is on the "Future of Health," alongside other sectors like edtech and SaaS. Their portfolio includes companies like MDisrupt, a digital health marketplace, and ConConnect, a social-impact network for justice-impacted individuals.

Zane Venture Fund could be a great match if you are an early-stage founder, particularly from an underrepresented background. Their focus on health, wellness, and mission-driven technology makes them a strong choice for founders in those spaces.

• Investment stages: Seed and early-stage venture
• Industries of focus: B2B, SaaS, health care, edtech, wellness
• Geographical presence: Atlanta, Georgia
• Founded: 2019
• Notable portfolio companies: MDisrupt, SkillCycle, ConConnect, SellerOS
• Number of investments: 20

You can refer to their website here.

6. Branded Hospitality

Branded Hospitality is a New York-based investment firm that focuses on the intersection of hospitality, technology, and media. They back companies that are building new solutions for the food and beverage and retail industries.

The firm's portfolio shows a strong focus on technology that modernizes restaurant and retail operations. Notable investments include Bbot, a QR-code payment platform acquired by DoorDash, and Chowly, which integrates delivery apps with restaurant POS systems.

Branded Hospitality is a good fit for founders building B2B software or technology for the hospitality sector. If your company offers a clear solution for restaurants, bars, or retail businesses, their industry expertise could be valuable.

• Investment stages: Seed, early-stage venture, private equity
• Industries of focus: Hospitality, food and beverage, retail technology, B2B software
• Geographical presence: New York City
• Founded: 2017
• Notable portfolio companies: Bbot, PourMyBeer, Ottonomy IO, Chowly, Big Chicken
• Number of investments: 73

You can refer to their website here.

7. 2048 Ventures

2048 Ventures is a New York-based, thesis-driven firm that invests in pre-seed and seed-stage companies. They partner with founders who are using science and technology to build businesses that address significant challenges.

The firm backs companies with deep technical foundations, with a portfolio spanning AI, robotics, and health care. Their investment in Aerodome, a drone startup acquired for over $300 million, highlights their ability to identify high-growth opportunities in specialized markets.

2048 Ventures is a good fit for early-stage founders with a background in science or engineering. If your company is building a business with a strong technical moat, this firm could be a valuable partner.

• Investment stages: Seed and early-stage venture
• Industries of focus: B2B, SaaS, AI, robotics, health care, biotechnology
• Geographical presence: New York City
• Founded: 2019
• Notable portfolio companies: Aerodome, GlossGenius, Soona, Nomic Bio, Vitally
• Number of investments: 136

You can refer to their website here.

8. Golden Section

Golden Section is a Houston-based firm that operates as both a venture capital fund and a founders studio. They partner with B2B SaaS companies, providing capital and hands-on support from the earliest stages.

A key aspect of their model is the combination of a traditional fund with a studio that helps build companies from the ground up. Their portfolio reflects a focus on capital-efficient B2B software businesses that aim for sustainable growth.

Golden Section is a strong potential partner for early-stage B2B SaaS founders who value operational involvement. Their studio model is particularly suited for entrepreneurs looking for a deeply engaged co-building relationship.

• Investment stages: Seed and early-stage venture, with debt options
• Industries of focus: B2B, SaaS, Software
• Geographical presence: Houston, Texas
• Founded: 2012
• Notable portfolio companies: MedScout, Podetize, Avise
• Portfolio size: 41 investments

You can refer to their website here.

9. Cervin Ventures

Cervin Ventures is a Palo Alto-based firm that invests in early-stage B2B technology companies. They partner with founders to help build and scale their businesses for long-term success.

The firm has a strong record in the enterprise software space, backing companies like feature-management platform LaunchDarkly. Their portfolio also includes successful exits like Punchh and EdCast, showing their ability to guide companies toward acquisition.

Cervin Ventures is a good match for founders of early-stage B2B software companies who are looking for an active partner. Their experience with enterprise technology makes them a strong choice for teams building for large organizations.

• Investment stages: Seed and early-stage venture
• Industries of focus: B2B, enterprise software, financial services
• Geographical presence: Palo Alto, California
• Founded: 2011
• Notable portfolio companies: LaunchDarkly, Punchh, EdCast, Tynker, Celona
• Number of investments: 85

You can refer to their website here.

10. Upekkha Vertical AI Accelerator

Upekkha is a Vertical AI Accelerator that helps Indian founders build global B2B software companies. While based in San Francisco, their core mission is to provide the capital and support needed for startups from India to enter and succeed in international markets.

The firm operates as both an accelerator and a venture capital fund, focusing on early-stage B2B SaaS companies. Their portfolio includes companies that have raised significant follow-on funding, such as skills-assessment platform iMocha and AI video-interviewing firm Talview, signaling a strong ability to prepare companies for growth.

Upekkha is an ideal partner if you are an Indian founder of an early-stage B2B SaaS company with global ambitions. Their accelerator model suggests a hands-on approach, making them a great fit for entrepreneurs seeking structured guidance on scaling internationally.

• Investment stages: Seed, Early Stage Venture
• Industries of focus: B2B, Enterprise Software, SaaS
• Geographical presence: San Francisco, California
• Founded: 2017
• Notable portfolio companies: Spyne, Talview, iMocha, Classpro
• Number of investments: 114

You can refer to their website here.

11. Signia Venture Partners

Signia Venture Partners is an early-stage firm in Redwood City that invests in founders building companies with the potential for significant global impact. They provide capital and support to entrepreneurs at the beginning of their business journey.

The firm's portfolio shows a focus on companies applying advanced technology to major industries like construction, climate, and health. Notable investments include construction-tech platform Inertia Systems and AI-driven weather forecaster Atmo.

Signia could be a strong partner if you are an early-stage founder using technology to address major challenges in large, established sectors. Their investments suggest a preference for businesses with a strong technical foundation and ambitious goals.

• Investment stages: Seed and early-stage venture
• Industries of focus: B2B, finance, climate tech, health tech, construction tech
• Geographical presence: Redwood City, California
• Founded: 2012
• Notable portfolio companies: Inertia Systems, Atmo, Kintsugi
• Number of investments: 117

You can refer to their website here.

12. Wildcat Venture Partners

Wildcat Venture Partners is a firm based in San Mateo, California, that invests in technology startups. They focus on B2B and B2B2C companies across several key markets, including digital health, edtech, and fintech.

The firm has a history of backing major enterprise software companies that achieve significant exits. Notable investments include Kabbage, a small-business lending platform acquired by American Express, and Marketo, a marketing-automation company purchased by Adobe.

Wildcat could be a good partner for founders of early-stage B2B or B2B2C companies in their focus sectors. Their experience with scaling companies to IPO or major acquisition makes them a strong choice for teams with high-growth ambitions.

• Investment stages: Seed, early, and late stage
• Industries of focus: B2B, enterprise SaaS, fintech, edtech, digital health
• Geographical presence: San Mateo, California
• Founded: 2015
• Notable portfolio companies: Kabbage, Marketo, Rocket Fuel, Ticketfly, Workday
• Number of investments: 39

You can refer to their website here.

What This Tells Us About The VC Scene for B2B Companies

This list shows a healthy and active investment community for B2B companies, with a notable concentration of firms focused on seed and early-stage rounds. For founders just starting out, this indicates a strong appetite for new ideas and a significant amount of available capital to help you build and find product-market fit.

While traditional tech hubs like the Bay Area and New York are well-represented, the presence of investors in cities like Atlanta, Philadelphia, and Houston shows growing geographic variety. The firms themselves are also diverse, from industry specialists in hospitality or fintech to those with unique operational models. This variety gives you more options to find a partner whose expertise truly aligns with your business goals.

Raise Confidently with Rho

Fundraising requires significant time and focus. We hope this list helps you connect with the right investors, so you can direct your energy where it matters most.

Once your round is closed, the work of building continues. If you’ve just raised, Rho can help you set up your financial stack in minutes.

Our platform gives you the tools to manage your new capital with confidence, offering integrated business banking, corporate cards, and bill pay built for startups.

FAQs about Venture Capital Firms Focused on B2B

Which B2B VC firms are in Boston?

Boston has a strong ecosystem for B2B startups. Firms like Underscore VC and Glasswing Ventures focus on enterprise technology and SaaS, providing capital and support for founders in the area looking to build and scale their companies.

What do Series A venture capital firms look for in B2B companies?

For a Series A round, B2B VC firms want to see strong product-market fit and a clear, repeatable sales model. You should have consistent recurring revenue, low customer churn, and a solid plan for scaling your operations.

Are there European VC firms that focus on B2B SaaS?

Yes, many European VC firms specialize in B2B SaaS. Investors like Point Nine in Berlin and Notion Capital in London have strong track records backing enterprise software companies, helping them grow within Europe and expand to global markets.

How should I approach B2B focused VC firms?

Research firms that align with your industry and stage. A warm introduction from a mutual connection is often best. Prepare a concise pitch deck that clearly outlines the problem you solve, your traction, and your team’s expertise.

What are some top fintech VC firms for B2B startups?

Several fintech venture capital firms invest in B2B. Firms like QED Investors and Ribbit Capital are well-known for backing innovative financial technology companies, from payment platforms to infrastructure software, often investing across multiple growth stages.

How can Rho help my startup after I raise funding?

Once your round is closed, our platform helps you manage your new capital. We provide integrated business banking, corporate cards, and automated bill pay, all designed to support your startup’s financial operations as you grow. Get started with Rho.